cotE [2019-07-02 09:03:17]

outer spore coat morphogenetic protein, controls the assembly of the outer spore coat layer

locus: BSU17030

pI: 4.14

mw: 20.83 kDa

protein length: 181 aa

gene length: 546 bp

function: assembly of the outer spore coat

product: spore coat morphogenetic protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.2|Class II]

Gene

Coordinates: 1,775,067 1,775,612

Phenotypes of a mutant

• mis-assembly of the outer spore coat [Pubmed|22171814]
• inactivation of ''[gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE]'' reduces sporulation efficiency to 49.1% that of wild type cells [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• assembly of the [protein|9C59B51FC19FC83BD14272008BD441833DD1738E|CotC]-[protein|EB690FA075EAC3E298C47AED082B5FC21E8F8F46|CotU] complex [Pubmed|20023017,18065538]

Protein family

• cotE family (according to Swiss-Prot)

[SW|Localization]

• encircles the forespore with a biased accumulation on the mother cell-proximal face of the forespore [Pubmed|23267091,22463703]
• outer spore coat, localization depends on [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|SpoIVA] [Pubmed|22773792,22171814]

Expression and Regulation

Operons

• genes: [gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE]
  description: [Pubmed|8760914]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,1691789], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|1691789], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: repression, [Pubmed|15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|1691789,15383836]

Biological materials

Mutant

• 1S105 ( ''cotE''::''cat''), [Pubmed|3139490], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S105&Search=1S105 BGSC]
• BKE17030 ([gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCGGCATGCCTCCTTG, downstream forward: _UP4_TAAAAAAGGGACTAGGGGAG
• BKK17030 ([gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCGGCATGCCTCCTTG, downstream forward: _UP4_TAAAAAAGGGACTAGGGGAG

labs

• [SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]
• [SW|Adriano Henriques], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]

References

Reviews

• 22192522,23202530,27227299

Original publications

• 8748030,17720779,10559171,8760914,15699190,7592342,3139490,8299942,1691789,12644503,17172339,19304857,11737650,12107147,20023017,18065538,22773792,15383836,22171814,23064347,23178679,23267091,22463703,21821751,24086406,25259857,25872412,26484546,26821119,26735940,27320701,28870294,30168214